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anti cd169  (Bio-Rad)


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    Structured Review

    Bio-Rad anti cd169
    Anti Cd169, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 184 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+anti+mouse+cd169/Rat+anti+Mouse+CD169/pmc13083911-9-0-2
    Average 95 stars, based on 184 article reviews
    anti cd169 - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Immunohistochemical staining:

    Article Title: CD169 is a marker for highly pathogenic phagocytes in multiple sclerosis.
    Article Snippet: .. Immunohistochemical stainings were performed as described previously.3,22 Antibodies used are as follows: mouse anti-human CD169 (Abcam, Cambridge, UK), rat anti-mouse CD169 (MOMA-1, Abd Serotec), rabbit anti-Iba1 (Wako, Neuss, Table 1. ..

    Staining:

    Article Title: HMGB1 couples LEF1 to regulate B cell immunity.
    Article Snippet: Cells were incubated at 4°C and detected with Attune NxT flow cytometer (Thermo Fisher Scientific) and analyzed with FlowJo V10 (Tree Star) software. .. To identify B cells and the MZ region, spleen sections were stained with Rat anti-Mouse CD169 (MCA947GA; Bio-Rad), followed by Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (A-11006; Thermo Fisher Scientific), to visualize the MZ; after 2 washes, spleen sections were stained with APC-anti-B220 (553092; BD Pharmingen) to demonstrate the B cells. .. Mice’s kidneys were cross-sectioned and the largest cross sections were embedded in OCT (4583; Sakura Finetek) and frozen in liquid nitrogen to prepare frozen sections.

    Article Title: Modifying macrophages at the periphery has the capacity to change microglial reactivity and to extend ALS survival.
    Article Snippet: 1Sorbonne Université, Institut du Cerveau Paris Brain Institute ICM, Inserm, CNRS, Paris, France.. 2Department of Neurological, Biomedical and Movement Science, University of Verona, Verona, Italy.. 3Genosplice, Paris, France.

    Blocking Assay:

    Article Title: Infectious Clones Produce SARS-CoV-2 That Causes Severe Pulmonary Disease in Infected K18-Human ACE2 Mice
    Article Snippet: .. Sections were blocked (Dako Serum-Free Protein Block; Dako) and immunolabeled with rat anti-mouse CD169 (AbD Serotec), rabbit anti-mouse cytokeratin 14 (Abcam), Alexa Fluor 647-conjugated rat anti-mouse Ly6G (BD Biosciences) antibodies, rabbit anti-mouse collagen type IV (Abcam), or rat monoclonal F4/80 (Abcam), and secondary antibody detection was performed using anti-rat Alexa Fluor 488 and anti-rabbit Alexa Fluor 546 antibodies (Thermo Fisher). .. Sections were washed and nuclei were labeled using Hoechst 33258 dye and mounted using ProLong Gold antifade (Thermo Fisher).

    Article Title: Infectious Clones Produce SARS-CoV-2 That Causes Severe Pulmonary Disease in Infected K18-Human ACE2 Mice
    Article Snippet: .. Sections were blocked (Dako Serum-Free Protein Block; Dako) and immunolabeled with rat anti-mouse CD169 (AbD Serotec), rabbit anti-mouse cytokeratin 14 (Abcam), Alexa Fluor 647-conjugated rat anti-mouse Ly6G (BD Biosciences) antibodies, rabbit anti-mouse collagen type IV (Abcam), or rat monoclonal F4/80 (Abcam), and secondary antibody detection was performed using anti-rat Alexa Fluor 488 and anti-rabbit Alexa Fluor 546 antibodies (Thermo Fisher). .. Sections were washed and nuclei were labeled using Hoechst 33258 dye and mounted using ProLong Gold antifade (Thermo Fisher).

    Immunolabeling:

    Article Title: Infectious Clones Produce SARS-CoV-2 That Causes Severe Pulmonary Disease in Infected K18-Human ACE2 Mice
    Article Snippet: .. Sections were blocked (Dako Serum-Free Protein Block; Dako) and immunolabeled with rat anti-mouse CD169 (AbD Serotec), rabbit anti-mouse cytokeratin 14 (Abcam), Alexa Fluor 647-conjugated rat anti-mouse Ly6G (BD Biosciences) antibodies, rabbit anti-mouse collagen type IV (Abcam), or rat monoclonal F4/80 (Abcam), and secondary antibody detection was performed using anti-rat Alexa Fluor 488 and anti-rabbit Alexa Fluor 546 antibodies (Thermo Fisher). .. Sections were washed and nuclei were labeled using Hoechst 33258 dye and mounted using ProLong Gold antifade (Thermo Fisher).

    Article Title: Infectious Clones Produce SARS-CoV-2 That Causes Severe Pulmonary Disease in Infected K18-Human ACE2 Mice
    Article Snippet: .. Sections were blocked (Dako Serum-Free Protein Block; Dako) and immunolabeled with rat anti-mouse CD169 (AbD Serotec), rabbit anti-mouse cytokeratin 14 (Abcam), Alexa Fluor 647-conjugated rat anti-mouse Ly6G (BD Biosciences) antibodies, rabbit anti-mouse collagen type IV (Abcam), or rat monoclonal F4/80 (Abcam), and secondary antibody detection was performed using anti-rat Alexa Fluor 488 and anti-rabbit Alexa Fluor 546 antibodies (Thermo Fisher). .. Sections were washed and nuclei were labeled using Hoechst 33258 dye and mounted using ProLong Gold antifade (Thermo Fisher).

    other:


    Article Title: VE-cadherin junction dynamics in initial lymphatic vessels promotes lymph node metastasis
    Article Snippet: Rat anti-mouse CD169 , Bio-Rad , MCA947G , AB_322322 , 1:50 (IF).

    Article Title: HMGB1 couples LEF1 to regulate B cell immunity
    Article Snippet: Sections were blocked and stained with Cy3-AffiniPure Donkey Anti-Mouse IgG (715-165-151; Jackson ImmunoResearch), imaged using a confocal microscope (LSM 780; Zeiss), and finally analyzed using the ZEN 2.3 software.



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    Image Search Results


    CD169+ macrophages in regenerating areas at 96-h and 192-h postinjury. CD169+ macrophages in the regenerating area of plantarflexor muscle group cross-sections at 96-h (A–D) and 192-h (E–H) postinjury. The regenerating area (black box) in each hematoxylin and eosin (H and E)-stained cross section (A, C, E, G) is matched to an area (black box) in a serial section stained with an anti-CD169 antibody (B, D, F, H) to detect the CD169 protein coded by Siglec1. h, hours postinjury

    Journal: Biological Research for Nursing

    Article Title: Estrogen Stabilizes Interferon-Inducible Genes During Skeletal Muscle Regeneration

    doi: 10.1177/10998004251411301

    Figure Lengend Snippet: CD169+ macrophages in regenerating areas at 96-h and 192-h postinjury. CD169+ macrophages in the regenerating area of plantarflexor muscle group cross-sections at 96-h (A–D) and 192-h (E–H) postinjury. The regenerating area (black box) in each hematoxylin and eosin (H and E)-stained cross section (A, C, E, G) is matched to an area (black box) in a serial section stained with an anti-CD169 antibody (B, D, F, H) to detect the CD169 protein coded by Siglec1. h, hours postinjury

    Article Snippet: The primary antibody was a purified rat anti-mouse CD169 antibody (AbD Serotec-Bio-Rad, 1:20 dilution, Hercules, CA; or Biolegend, 1:50 dilution, San Diego, CA).

    Techniques: Staining